Analyte
Analyte An Analyte is the substance or chemical constituent that ... procedure. For instance, in an immunoassay, the analyte may be the ligand or the binder, while in blood glucose testing, the analyte is glucose.
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Capillary electrophoresis
... migration velocity ($u\_p$) of an analyte toward the electrode of opposite charge is ... solution. The frictional forces experienced by an analyte ion depend on the viscosity (η) of ... 1 Accordingly, the electrophoretic mobility of an analyte at a given pH is given by ... math> is the net charge of the analyte and $r$ is the Stokes radius of the analyte. The Stokes radius is given by: < ...
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Electrospray ionization
... substance which is to be studied, the analyte, dissolved in a large amount of solvent ... is usually much more volatile than the analyte. Volatile acids, bases or buffers are often ... added to this solution as well. The analyte exists as an ion in solution either ... now suspended in air evaporate, the charged analyte molecules are forced closer together. The proximity ... is the repulsive Coulombic forces between charged analyte molecules that drive it. This process ...
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High performance liquid chromatography
... frequently in biochemistry and analytical chemistry. The analyte is forced through a column of the ... to assist in the separation of the analyte components. Advances in the HPLC technology have ... 50% methanol (depending on how hydrophobic the analyte is) over 25 minutes. The gradient separates the analyte mixtures as a function of how well the changing solvent mobilizes the analyte. In other words, it is magic. ...
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Spectroscopy
... is typically used to produce excitation of analyte atoms. Since analyte atoms are excited by the heat of ... the sample, but the excitation of the analyte atoms is done by the use of ... at various wavelengths for each type of analyte. In AA, the amount of light absorbed ... through the flame determines the amount of analyte in the sample. A graphite furnace ...
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Chromatography
... the sample, a mixture which contains the analyte, in the "mobile phase", often in a ... achieved when the retention time of the analyte differs from that of other components in ... is based on a partition equilibrium of analyte between a liquid stationary phase and a ... frequently in biochemistry and Analytical Chemistry. The analyte is forced through a column (stationary phase ... peaks). Another way to decrease time the analyte stays on the column is to ...
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Microdialysis
... into account. The absolute concentration of an analyte (the substance or substances being analysed) in ... a relatively high flow rate, then the analyte will most likely not fully equilibrate between ... dialysate will underestimate the concentration of the analyte in the ECF. There are a number ... models available for quantifying the fraction of analyte in the dialysate, with Bungay et. al ... field have gone towards increasing the recovered analyte concentration in the dialysate. This can ...
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Electron ionization
... in the ionization space, they interact with analyte molecules in the gas phase, causing them ... which can convey structural information about the analyte. The efficiency of ionization and production of ... depends strongly on the chemistry of the analyte and the energy of the electrons. At ... the interactions between the electrons and the analyte molecules do not transfer enough energy to ... 14 nm), and energy transfer to organic analyte molecules is maximized, leading to the ...
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Countercurrent chromatography
... eliminating solid supports, permanent adsorption of the analyte onto the column is avoided, and a near 100% recovery of the analyte can be achieved. The instrument is also ... them has a large area, and the analyte can move between the phases according to ... is a ratio of the amount of analyte found in each of the solvents at equilibrium, and is related to the analyte's affinity for one over the ...
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Gas-liquid chromatography
... a known volume of gaseous or liquid analyte is injected into the entrance of the ... microsyringe. Although the carrier gas sweeps the analyte molecules through the column, this motion is inhibited by the adsorption of the analyte molecules either onto the column walls or ... of progression, the various components of the analyte mixture are separated as they progress along ... and by the residence time of the analyte in the column. Two types of ...
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